The article presents a description of the extracting process of biologically active substances from pine bark. It also provides a schematic diagram of an experimental setup for extracting biologically active substances. It describes the methodology for conducting experimental studies on the extraction of biologically active substances. The authors present the dependence of the concentration of biologically active substances (coniferous wax, anthocyanidin dye, and pectin substances) from the type and concentration of the extractant, water duty, temperature, and time. The article also demonstrates the extraction of maximum amounts of coniferous wax, anthocyanidin dye and pectin under specific conditions.